Journal of Integral Sciences

Molecular Genotyping, 16S rRNA Sequencing, and Evolutionary Matrix of Streptomyces sp. Strain ANU-27 Isolated from Estuarine Mangrove Sediments

2026-07-20T04:10:12-04:00

Estuarine mangrove environments are unique, highly dynamic habitats harboring a rich and unexplored diversity of bioactive actinobacteria. In this study, we highlight the extensive molecular identification, genetic sequencing, and evolutionary positioning of the bacterial isolate ANU-27, which exhibited superior broad-spectrum antimicrobial efficacy during initial phenotypic screenings. Genomic DNA was successfully extracted using the CTAB-lysozyme methodology, yielding634±32 µg/gof high-integrity DNA with a distinct genomic G+C content of 55.1% calculated via thermal denaturation midpoint (T_m= 92.3C).The 16S rRNA gene locus was targeted and amplified via PCR utilizing 27F and 1492R universal primers, generating a sharp ~1500 bp amplicon. Bidirectional Sanger sequencing resolved a definitive sequence spanning 1177 nucleotides with a high ribosomal G+C ratio 59.13%. Comprehensive homology mapping via NCBI BLASTn paired with a1000-replicate Maximum Parsimony cladogram clustered the strain with 100% sequence identity( E-Value = 0.0) within the genus Streptomyces, revealing an immediate phylogenetic node shared with Streptomyces maritimus strain SBK2-IR9 and Streptomyces rochei strain ABU8. These findings firmly clarify the taxonomic assignment of strain ANU-27, spotlighting its high secondary metabolic and biotechnological promise.

Synthesis, spectroscopic characterization, and combined DFT/GFN2-xTB study of ethyl 4-[2-(3-hydroxy-4-methoxyphenyl)-4,9-dioxo-2,3,4,9-tetrahydro-1,3-benzoxazepin-3-yl] benzoate

2026-08-25T05:06:14-04:00

A new seven-membered oxygen/nitrogen heterocycle, ethyl 4-[2-(3-hydroxy-4-methoxyphenyl)-4,9-dioxo-2,3,4,9-tetrahydro-1,3-benzoxazepin-3-yl]benzoate (2), was synthesized in two steps from isovanillin and benzocaine. Acid-catalyzed condensation produced azomethine 1, followed by [2+5] cycloaddition with phthalic anhydride to form the seven-membered ring without loss of a small molecule. Both compounds were characterized by FT-IR and ¹H/¹³C NMR spectroscopy. Formation of 2 was confirmed by disappearance of the azomethine band at 1642 cm⁻¹ and appearance of lactone and lactam carbonyl bands at 1709 and 1674 cm⁻¹, respectively. The phenolic O–H bands shifted from 3369 cm⁻¹ in 1 to 3213 and 3113 cm⁻¹ in 2, indicating stronger hydrogen bonding. The azomethine carbon resonance at 162.1 ppm was replaced by lactam and lactone carbonyl signals at 174.9 and 169.2 ppm. Computational studies using B3LYP/6-31G(d), supported by ETKDG, MMFF94, and GFN2-xTB conformational analysis, showed that the heterocycle adopts a twist-boat conformation with a Cremer–Pople puckering amplitude of 0.892 Å. The HOMO–LUMO gap was 4.331 eV, with chemical hardness of 2.166 eV and electrophilicity of 3.241 eV. The molecule exhibits strong electrophilic and nucleophilic characteristics, with an electrostatic potential ranging from −38.0 to +45.3 kcal mol⁻¹.

Structure-Performance Relationships in Coupling Reagents: A Review of DFT- and QSPR-Guided Design of Azo Chromogens for Drug Determination

2026-08-25T05:06:08-04:00

Diazotization followed by azo coupling is still one of the most heavily used derivatization routes in pharmaceutical UV-visible spectrophotometry, and yet the selection of the coupling reagent is very often made by trial and error, or simply by copying whatever the previous paper in the same journal happened to use. This review asks a narrower question than the usual survey of applications: what structural features of a coupler actually control the analytical figures of merit, and how far can density functional theory (DFT) and quantitative structure-property relationships (QSPR) replace that guesswork with prediction? We revisit the coupling step as an electrophilic aromatic substitution whose rate, regiochemistry and pH window are set by the ionization state of the coupler and by the electrophilicity of the arenediazonium ion generated from the drug. We then connect those variables to the quantities an analyst actually cares about: absorption maximum, molar absorptivity and colour stability. Conceptual-DFT indices, in particular the electrophilicity index, the nucleophilicity index and condensed Fukui functions, are shown to rationalize reagent behaviour that is otherwise reported as an empirical observation. Time-dependent DFT reproduces azo absorption maxima to within roughly 6-12 nm when the functional and the solvation model are chosen with care, while recent QSPR and machine-learning models predict λ_max across hundreds of azo dyes with external coefficients of determination approaching 0.9. A structure-performance map of the common couplers is assembled, recurring errors in present computational practice are identified, and a minimum reporting set is proposed.

DESIGN AND OPTIMIZATION OF AIR SUSPENSION MICROENCAPSULATION PROCESS

2026-06-16T14:22:55-04:00

Microencapsulation is a versatile and well-established technology used to entrap solid, liquid, or gaseous core materials within a protective coating to enhance stability, control release, and improve handling properties. The analyzed articles collectively highlight the fundamental principles, materials, techniques, and applications of microencapsulation across pharmaceutical, food, agricultural, textile, and construction industries. Various core and coating materials, including polymers, proteins, and lipids, are discussed with emphasis on their compatibility, biodegradability, and release characteristics. The articles also address microcapsule morphology, release mechanisms, and factors influencing encapsulation efficiency. Despite its advantages, challenges such as high production costs, scalability issues, and non-uniform particle size are noted. Overall, the reviewed literature demonstrates that microencapsulation is a critical enabling technology with significant potential for innovation, especially when optimized materials and methods are selected to meet specific application requirements.

NOVEL NANO PARTICLES APPROACHES IN TRAGETED THERAPY OF BRAIN CANCER

2026-06-17T22:10:20-04:00

Brain cancer, particularly glioblastoma, remains one of the most aggressive and difficult-to-treat malignancies due to the restrictive nature of the blood–brain barrier (BBB) and blood–brain–tumour barrier (BBTB). The reviewed articles collectively highlight the transformative role of nanotechnology in improving the diagnosis, imaging, and treatment of brain tumours. Nanoparticles (NPs), including lipid-based, polymeric, metallic, carbon-based, virus-based, and biomimetic nanomaterials, demonstrate enhanced permeability, targeted delivery, and multifunctional capabilities. Their nanoscale size, high surface area, and modifiable surfaces enable efficient BBB penetration, tumour-specific targeting, drug encapsulation, and controlled release. In addition to therapeutic applications, nanostructures offer optical, magnetic, and photodynamic properties that significantly enhance imaging precision and multimodal diagnostics. While nanotechnology presents substantial clinical promise, concerns regarding neurotoxicity and long-term. safety necessitate standardized toxicological evaluation. Current research emphasizes improving specificity, minimizing adverse effects, and developing biocompatible and cell membrane-coated systems for safer clinical translation. Overall, nanotechnology-based platforms represent a rapidly advancing strategy that integrates targeted therapy and advanced imaging, offering improved efficacy and potential breakthroughs in brain cancer management while underscoring the importance of safety optimization.

ORGAN ON A CHIP INNOVATION IN PRECISION MEDICINE AND DRUG DESIGN

2026-06-17T22:19:33-04:00

Organ-on-a-chip (OOC) technology is an advanced microengineered system that recreates the structure and function of human organs on a small, chip-based platform. By combining cell biology, biomaterials, engineering, and microfluidics, these devices imitate the natural environment of tissues, including fluid flow, mechanical forces, and cell interactions. Unlike traditional 2D cell cultures and animal testing, OOC systems provide more realistic physiological conditions while reducing cost and ethical concerns.Various organ models such as lung, liver, kidney, heart, intestine, skin, and brain have been successfully developed. These systems are widely used in drug screening, toxicology studies, disease modeling, pharmacokinetics, and personalized medicine research.

THERAPEUTIC POTENTIAL OF PHYTO CHEMICALS IN DIABETES CARE

2026-06-17T22:37:37-04:00

The Therapeutic possibilities of plant based treatments the management of diabetetes of mechanism of the action. Diabetes mellitus is a common chronic Illness that serious danger at around the entire world. the properties of following plants are Alstonia scholaris, Annona squamosa, momordica charantia, cinnamon, fenugreek, Allium sativum, Neem, Ocimum sanctum, Ginseng berry, Aloe vera this data process are identify potiential phytoconstituents for treatment of diabetes mellitus.Herbal drugs are controlling diabetes safely effectively and different methods of action experiments on animals and humans. This review important discoveries and medicinal uses herbal treatment for diabetes WHO acknowledges the potiential of herbal drugs in treating different health issues, such as diabetes utilized conventional therapies. It is important to emphasize that herbal treatments are displayed potential diabetes they should be healthcare experts.herbal remedies have been traditionally treat to diabetes.the parts used test models hypoglycaemic [blood-sugar-lowering] active compounds of 20 plant species for 12 families crucial role of plant diabetes. The plants can delay complications from diabetes and metabolic imbalance.This review can attempt to cover for herbal plants like melon, fenugreek, ginseng cinnamon, garlic, gymnema managing blood glucose level due to saponin, terpenoids,flavonoids useful controlling diabetes descrption of gymnema gurmarin possible mechanism the gumarin helps manage taste buds receptors TIR1,TIR3 both β-cells and islets, PPARγ (peroxisome proliferator activated receptor gamma) β-cells is stimulated by gymnema. Plant culture methods gymnemic acid production Diabetes mellitus affecting over 537 million people poses health challenges prevalence and complications like Neuropathy, cardiovascular sylvestre, momordica charantia, trigonella, foenum-graecum cinnamomum veum, Aloe vera and clinical efficacy through results vary due to standardization issues.

HE POWDER OF PLANTS: EXPLORING HERBAL NUTRACUETICLES

2026-06-17T22:40:05-04:00

Nutraceuticals are bioactive compounds derived from food sources that provide health benefits beyond basic nutrition. This seminar report presents an extensive overview of nutraceuticals, including their definition, classification, advantages, bioactive components, probiotics, safety considerations, regulatory frameworks, and future prospects. The increasing prevalence of lifestyle-related disorders has created demand for preventive healthcare strategies, where nutraceuticals play a vital role. Functional foods, dietary supplements, antioxidants, and probiotic-based products contribute to maintaining physiological balance and improving quality of life. Advances in nanotechnology, nutrigenomics, and microbiome research are shaping the future of nutraceutical development. This report highlights the importance of scientific validation, quality control, and standardized regulatory practices to ensure safety and efficacy in modern healthcare.